cardiac fibroblasts (CF) is the principal extracellular matrix-producing cells. In response to injury, CF transdifferentiate into myofibroblasts. Intracellular calcium (Ca2 +) signals, are involved in fibroblast proliferation and differentiation, activated in fibroblasts via the transient receptor potential (TRP) channels, but the function of this channel has not been studied in human ventricular CF. Under evaluation in this study, is the role of TRP channels in the differentiation of human-induced ventricular CF by changing the growth factor beta (TGF-β), a pro-fibrotic cytokine.Human CF ventricular used in this study.
CF to myofibroblast differentiation induced by TGF-β and identified by the expression of smooth muscle actin.Results showed that Ca2 + signaling is an important component of differentiation CF ventricle. CF treated with TGF-β showed an increased expression of the channel TRP, TRPV4, both at the mRNA and protein levels, which are associated with CF-myofibroblast trans-differentiation, as evidenced by the upregulation of actin muscle α-smooth, a myofibroblast marker, and plasminogen activator inhibitor -1, which is a marker of fibrogenesis.
TRPV4 agonist-induced conversion of CF into myofibroblasts, while it is also an antagonist of Ca2 + chelating agents is reduced, showing that Ca2 + influx is required for CF throughTRPV4 trans-differentiation. Overall, these results suggest that TRPV4-mediated Ca2 + influx participate in regulating differentiation into myofibroblasts CF human ventricle through the MAPK / ERK pathway.
Overall, these results suggest that TRPV4-mediated Ca2 + influx participate in regulating differentiation into myofibroblasts CF human ventricle through pathways MAPK / ERK.
Transient receptor potential channel TRPV4 mediates TGF-β1-induced differentiation of human ventricular fibroblasts.
Effect of dual inhibition of Ras-MEK-ERK pathway and GSK3β on developing rabbit embryos cultured in vitro.
Dual inhibition (2i) of the pathway Ras-MEK-ERK and GSK3β allowing the derivation of embryonic stem cells (ESCs) from mouse strain flame retardant and, for strains of permissive, allowing the ESC derivation with no involvement of protein factors external stimuli. Additionally, blocking ERK signals in the 8-cell-stage mouse embryos leads to ablation GATA4 / 6 expression in hypoblasts, showing fibroblast growth factor (FGF) dependence hypoblast formation in mouse.
In humans, cattle or pig embryo, hypoblast remains unaffected or show little to moderate decrease in the number of cells. In this study, we showed that the separation of the hypoblast and epiblast in embryonic rabbit is independent FGF and 2i treatment elicits only limited gains in favor of OCT4-positive epiblast population to population hypoblast GATA4- / 6-positive. Previously been shown that TGFβ / Activin A inhibition overcome pervasive and inhomogeneity differentiation of iPSCs rat, mouse ESCs and iPSCs humans while encouraging them to acquire naive nature.
However, TGFβ / Activin A inhibition, alone or together with a Rho-associated, coiled-coil containing protein kinase (ROCK) inhibition, are not compatible with the survival of rabbit embryos by preimplantation rabbit embryos ultrastructural analysis by electron microscopy. In a rabbit model of ovulation in mating allows the exact timing of the development of the pregnancy.
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:50-1:200
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:200-1:1000, IHC:1:100-1:300
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100
Description: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Description: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Description: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Description: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against FGFRL1. Recognizes FGFRL1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: Human FGFRL1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.
Description: Description of target: Fibroblast growth factor receptor-like 1 is a protein that in humans is encoded by the FGFRL1 gene. It is mapped to 4p16.3. The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml
Description: Description of target: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.051 ng/mL
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FGFRL1 (N-term). This antibody is tested and proven to work in the following applications:
Description: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FGFRL1 - middle region. This antibody is tested and proven to work in the following applications:
It produces some desired stage embryos in the pregnancy and a relatively short gestation period, making the rabbit embryo find a suitable model for cellular functions and mechanisms of maintenance of pluripotency in embryonic cells and embryonic stem cells derived from other mammals.
Felicia
